Protocol: Dot Blot Checkerboard Titration of Antibodies

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Dot blots are similar to Western blots, however the proteins are not separated electrophoretically prior to transfer to a membrane but are instead spotted

This video gives details about the two techniques that is dot blot and slot blot in notes form It Typical applications include clone screening with DNA RNA probes in Southern Northern blots and immunological screening with antibodies in Western blots

african slot png A dot blot is a technique in molecular biology used to detect proteins It represents a simplification of the western blot method, with the 2 Other RNA size markers -- 5 Blotting the gel -- 6 Further reading -- 7 References -- CHAPTERS Dot and slot blotting